Molecular and Cellular Biology

Biology 351

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  • Solutions and Units of Measure
  • DNA isolation
  • Restriction Enzyme Digest
  • Making Nonradioactive Probes
  • Southern Transfer
  • Southern Analysis

  • Bacterial Transformation
  • Plasmid Miniprep
  • Cycle Sequencing
  • Database Search Tools

  • RNA Isolation
  • RNA Electrophoresis

    Your DIG-probe is a ~350 bp product and below you will find specifics on the marker.

    marker info

    23Kb

    9.4Kb

    6.6Kb

    4.4Kb (faint)

    2.3Kb

    2.0Kb

    0.5Kb (faint)

    Here are your bioinformatics sequences:

    >rrm-1
    IYVGNLMFQVTDKDIAREFSQQGRFVYIHLKSGRVTGRRDGFGYILFTTY KDAHQALSTLGGFYVNSRNLSC

    >rrm-2
    LYIRNLDTVTEKQSMLTEFSPFGDDLHLRAIKNSDASSSANSKGFAFIDF KDEDSAEAAFRKLGEIPINGSTLDV

    >rrm-3 (Psi-blast)
    AYVSRLSYDTTEEHVREAFGNFPPIVSALLINDCNNFGFIEFKDREETAH IMEGLPKTDFNNVSVKK

    >rrm-4
    LFIIGLPDETSESQLRQIFAAFGGVCTFNLIKQPVTRLTSERNKCRGYAF VIYQNKEDAANARNLNGIAINNRIVRV

    >rrm-5
    LYVGDVSYQIQTTSVLIYFSPEGDKTTISMILQKNNDGKGFVHFPKHPSA TDIPDRMDGSELLGKKLAL

    >rrm-6
    YIGGLAEKINKSELSLLFGRFGSSMSAKVVKIRQTNRNQPMCFISFKTEE DTNMAMKEMFNKEFLGCELDV

    >rrm-7
    LFVKNLEPMETTDEDVRAFFTKNGVLKECNMIVDKETGHLRGKGALNFED PVSAEKAVRNLNGGKFNSNEATL

    >rrm-8
    VKIRGLDQGATGKAIRDAFNQYGNLIVIIVVMDTNSRQFNMGFIEFKDQL DANVALREKDGIPVLNERSAV

    >rrm-9(good seq for testing BLOSSUM matrices)
    LYIGDLPDAIRDDDLKDLFRKFGEIETIKAISSGVPTRARGYAFVRFARY EDIREALDEKEGYQPCDYAEKI

    >rrm-10
    LFLSNLPRDCNRHKLRDMFSKYGLIVKIRAHPDKDTGPTRGYAFVQFKSS EDVDKAKALNKYFLGLRKIRV

    PHI Pattern: GFP (SYG) residues 65-67 in Aequorea victoria
    >gi|18175269|gb|AAK02065.1| GFP
    MSKGEELFTGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIKFICTT GKLPVPWPTLVTTLSYGILCFARYPEHMKMNDFFKSAMPEGYIQERTIFF QDDGKYKTRGEVKFEGDTLVNRIELKGMDFKEDGNILGHKLEYNFNSHNV YIMPDKANNGLKVNFKIRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHY LSTQTAISKDRNETRDHMVFLEFFSACGHTHGMDELYK

    Sequences for Clustalw, Mview and Jalview Mirochondrial Porin sequences

    >PORIN_HUMAN
    MSWCNELRLPALKQHSIGRGLESHITMCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEI AIEDQICQGLKLTFDTTFSPNTGKKSGKIKSSYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSAKSKLTRNNFAVGYRTGDFQLHTNVNDGTEFGGSIYQ KVCEDLDTSVNLAWTSGTNCTRFGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSINAGGHKVGSPWSWRLNPAERNLWEWISEDLALIYFHCDQ QQAFFPPEDDQNKG

    >POR1_MOUSE_14_295
    MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVNGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFDSSFSPNTGKKN AKIKTGYKREHINLGCDVDFDIAGPSIRGALVLGYEGWLAGYQMNFETSKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIA AKYQVDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQ

    >POR3_BOVIN_1_282
    MCNTPTYCDLGKAAKDVFNKGYGFGMVKIDLRTKSCSGVEFSTSGHAYTDTGKASGNLETKYKICNYGLTFTQKWNTDNTLGTEISWENKLAEGLKLTLDTIFVPNTGKKS GKLKASYKRDCFSLGSNVDIDFSGPTIYGWAVLAFEGWLAGYQMSFDTAKSKLSQNNFALGYKAADFQLHTHVNDGTEFGGSIYQKVNEKIETSINLAWTAGSNNTRFGIA AKYKLDCRTSLSAKVNNASLIGLGYTQTLRPGVKLTLSALIDGKNFNAGGHKVGLGFELE

    >PORI_DROME_1_281
    MAPPSYSDLGKQARDIFSKGYNFGLWKLDLKTKTSSGIEFNTAGHSNQESGKVFGSLETKYKVKDYGLTLTEKWNTDNTLFTEVAVQDQLLEGLKLSLEGNFAPQSGNKNG KFKVAYGHENVKADSDVNIDLKGPLINASAVLGYQGWLAGYQTAFDTQQSKLTTNNFALGYTTKDFVLHTAVNDGQEFSGSIFQRTSDKLDVGVQLSWASGTSNTKFAIGA KYQLDDDASVRAKVNNASQVGLGYQQKLRDGVTLTLSTLVDGKNFNAGGHKIGVGLELE

    >POR4_SOLTU_3_274
    GPGLYTEIGKKARDLLYKDYQSDHKFSITTYSPTGVVITSSGSKKGDLFLADVNTQLKNKNVTTDIKVDTNSNLFTTITVDEAAPGLKTILSFRVPDQRSGKLEVQYLHDY AGICTSVGLTANPIVNFSGVVGTNIIALGTDVSFDTKTGDFTKCNAGLSFTNADLVASLNLNNKGDNLTASYYHTVSPLTSTAVGAEVNHSFSTNENIITVGTQHRLDPLT SVKARINNFGKASALLQHEWRPKSLFTVSGEVDTKSVDKGAKFGLALALK

    >POR1_WHEAT_1_274
    MGGPGLYSGIGKKAKDLLYRDYQTDHKFTLTTYTANGPAITATSTKKADLTVGEIQSQIKNKNITVDVKANSASNVITTITADDLAAPGLKTILSFAVPDQKSGKVELQYL HDYAGINASIGLTANPVVNLSGAFGTSALAVGADVSLDTATKNFAKYNAALSYTNQDLIASLNLNNKGDSLTASYYHIVEKSGTAVGAELTHSFSSNENSLTFGTQHTLDP LTLVKARINNSGKASALIQHEFMPKSLCTISAEVDTKAIEKSSKVGIAIALK

    >POR6_SOLTU_3_274
    GPGLYSDIGKKARDLLYRDYVSDHKFTVTTYSTTGVAITASGLKKGELFLADVSTQLKNKNITTDVKVDTNSNVYTTITVDEPAPGLKTIFSFVVPDQKSGKVELQYLHEY AGINTSIGLTASPLVNFSGVAGNNTVALGTDLSFDTATGNFTKCNAGLSFSSSDLIASLALNDKGDTVSASYYHTVKPVTNTAVGAELTHSFSSNENTLTIGTQHLLDPLT TVKARVNSYGKASALIQHEWRPKSLFTISGEVDTRAIEKSAKIGLAVALK

    >POR1_ARATH_3_274
    GPGLYTEIGKKARDLLYKDHNSDQKFSITTFSPAGVAITSTGTKKGDLLLGDVAFQSRRKNITTDLKVCTDSTFLITATVDEAAPGLRSIFSFKVPDQNSGKVELQYLHEY AGISTSMGLTQNPTVNFSGVIGSNVLAVGTDVSFDTKSGNFTKINAGLSFTKEDLIASLTVNDKGDLLNASYYHIVNPLFNTAVGAEVSHKLSSKDSTITVGTQHSLDPLT SVKARVNSAGIASALIQHEWKPKSFFTISGEVDTKSIDKSAKVGLALALK

    >PORI_NEUCR_1_282
    MAVPAFSDIAKSANDLLNKDFYHLAAGTIEVKSNTPNNVAFKVTGKSTHDKVTSGALEGKFTDKPNGLTVTQTWNTANALETKVEMADNLAKGLKAEGIFSFLPATNARGA KFNLHFKQSNFHGRAFFDLLKGPTANIDAIVGHEGFLAGASAGYDVQKAAITGYSAAVGYHAPTYSAAITATDNLSVFSASYYHKVNSQVEAGSKATWNSKTGNTVGLEVA TKYRIDPVSFVKGKINDRGVAAIAYNVLLREGVTLGVGASFDTQKLDQATHKVGTSFTFE

    >POR1_YEAST_3_281
    PPVYSDISRNINDLLNKDFYHATPAAFDVQTTTANGIKFSLKAKQPVKDGPLSTNVEAKLNDKQTGLGLTQGWSNTNNLQTKLEFANLTPGLKNELITSLTPGVAKSAVLN TTFTEPFFTARGAFDLCLKSPTFVGDLTMAHEGIVGGAEFGYDISAGSISRYAMALSYFAKDYSLGATLNNEQITTVDFFQNVNAFLQVGAKATMNCKLPNSNVNIEFATR YLPDASSQVKAKVSDSGIVTLAYKQLLRPGVTLGVGSSFDALKLSEPVHKLGWSLSF

    >DinoDNA "Dinosaur DNA" from Crichton's JURASSIC PARK p. 103 nt 1-1200
    GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGC TGCTCACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTG CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA AGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACT CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCT GGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGG CCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA CGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCG CACATGGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGG CTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACACGACTTAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCC GCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGG CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGG CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCT

    >DinoDNA "Dinosaur DNA" from Crichton's THE LOST WORLD p. 135
    GAATTCCGGAAGCGAGCAAGAGATAAGTCCTGGCATCAGATACAGTTGGAGATAAGGACG GACGTGTGGCAGCTCCCGCAGAGGATTCACTGGAAGTGCATTACCTATCCCATGGGAGCC ATGGAGTTCGTGGCGCTGGGGGGGCCGGATGCGGGCTCCCCCACTCCGTTCCCTGATGAA GCCGGAGCCTTCCTGGGGCTGGGGGGGGGCGAGAGGACGGAGGCGGGGGGGCTGCTGGCC TCCTACCCCCCCTCAGGCCGCGTGTCCCTGGTGCCGTGGGCAGACACGGGTACTTTGGGG ACCCCCCAGTGGGTGCCGCCCGCCACCCAAATGGAGCCCCCCCACTACCTGGAGCTGCTG CAACCCCCCCGGGGCAGCCCCCCCCATCCCTCCTCCGGGCCCCTACTGCCACTCAGCAGC GGGCCCCCACCCTGCGAGGCCCGTGAGTGCGTCATGGCCAGGAAGAACTGCGGAGCGACG GCAACGCCGCTGTGGCGCCGGGACGGCACCGGGCATTACCTGTGCAACTGGGCCTCAGCC TGCGGGCTCTACCACCGCCTCAACGGCCAGAACCGCCCGCTCATCCGCCCCAAAAAGCGC CTGCTGGTGAGTAAGCGCGCAGGCACAGTGTGCAGCCACGAGCGTGAAAACTGCCAGACA TCCACCACCACTCTGTGGCGTCGCAGCCCCATGGGGGACCCCGTCTGCAACAACATTCAC GCCTGCGGCCTCTACTACAAACTGCACCAAGTGAACCGCCCCCTCACGATGCGCAAAGAC GGAATCCAAACCCGAAACCGCAAAGTTTCCTCCAAGGGTAAAAAGCGGCGCCCCCCGGGG GGGGGAAACCCCTCCGCCACCGCGGGAGGGGGCGCTCCTATGGGGGGAGGGGGGGACCCC TCTATGCCCCCCCCGCCGCCCCCCCCGGCCGCCGCCCCCCCTCAAAGCGACGCTCTGTAC GCTCTCGGCCCCGTGGTCCTTTCGGGCCATTTTCTGCCCTTTGGAAACTCCGGAGGGTTT TTTGGGGGGGGGGCGGGGGGTTACACGGCCCCCCCGGGGCTGAGCCCGCAGATTTAAATA ATAACTCTGACGTGGGCAAGTGGGCCTTGCTGAGAAGACAGTGTAACATAATAATTTGCA CCTCGGCAATTGCAGAGGGTCGATCTCCACTTTGGACACAACAGGGCTACTCGGTAGGAC CAGATAAGCACTTTGCTCCCTGGACTGAAAAAGAAAGGATTTATCTGTTTGCTTCTTGCT GACAAATCCCTGTGAAAGGTAAAAGTCGGACACAGCAATCGATTATTTCTCGCCTGTGTG AAATTACTGTGAATATTGTAAATATATATATATATATATATATATCTGTATAGAACAGCC TCGGAGGCGGCATGGACCCAGCGTAGATCATGCTGGATTTGTACTGCCGGAATTC

    Complete cDNA sequences: 

    Assembly sequence for sample 77-2-12-G01, no significant match,from R to F: 
Composition  288  A; 323  C; 362  G; 244  T; 0 OTHER
Percentage:  24%  A; 27%  C; 30%  G; 20%  T; 0%OTHER
Molecular Weight (kDa): ssDNA: 376.76    dsDNA: 750.4
1      TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGG GTGGGTCGAT CACAAGTTTG 
61     TACAAAAAAG CAGGCTTGTC GACTCGCTTT GCTCGTCACA TCGCGTTCTC AAGGTGTCCG 
121    TCCGTCAACT CGAGGTGCAA GATGATGACA AAGATGACGC TGCTCACGCT GATCGTGTGC 
181    GCGGGCGCCG TGCCCGTCGG CCCCAACTCG GAGGTTGTCC AGAACTCGGA GCTTGCTGGC 
241    GACGCGCAGC CCGGTCTCGC CCTGCAGTTC GGTCCCGGTC CTACTCTGGG CGGCAGTGGC 
301    GACTATGACT GCGGGTGGAG CTCTGCAGAC TTTGGCGAGT ATTGCACCGT GCCGCTTACC 
361    GACGCGCAAG CAAAGGCCAT CTACAACCAC TACAAACCAC CCCTGGGGGG TGATGATTCA 
421    GTGGAGGGAT GGAACACGAA TTGCATGAGG TATTGCACCA AGGACCAACC TTCTAATTCT 
481    AAGTGCGGAA ATGGAATGCA CGGCCAATGC TACGGGGGAG ATGTGTGCGT CAACCGTCAC 
541    ATCGTCACAG GTGCCTGGTA CAGCAAGTGC AAATCGACAG ATGGCATTCC GGAGTTTGAT 
601    CCACCGACTC CCCCGTCTCC GGATGAGTGC ATTGCTAACC ACTGCAAGGG CGGTTGCCTC 
661    CAGCCGGATA CGCAGAATCC GGGCAAGCAG GTTTGCCACA CCGAAGGCCT TGGTCCGGAT 
721    GCAGAGGATC ACTGCAAACA ACATCACTTT ACGTGGTGCC CGGCGGAGTG AAGGCATCGC 
781    CGCATGCAGC ATGCAACCGC GTGCCATCGT CTCTAACGAC CCATCGGACC CATCCGACCA 
841    AGATATGGGG GCGAGATGAG TGGGCGCTGA ACACTAACTG TGCGTGAGGG TGGATGGGTA 
901    CTCGGAATGA GGAGTGTCTG ATACGTTGTG GCAGTCTAGC GTGCGTGCAG CGGTAGCTCA 
961    GAGCCAGCTC TTGTCTGTCT GGGTTGCAGT ATCGGGGCGC GGGATCGGGG TTCGGCGCGG 
1021   GGTCGTGATG TTCCGGGCTC GCTGTCCATT CCCCTCGCCT AACGTGACGA AATGGGTACT 
1081   ACATGTTTAT ATACAGTCAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAG GGCGGCCGCA 
1141   CGCGTACCCA GCTTTCTTGT ACAAAGTGGT GACGTCGAGC TCTAAGTAAG TAACGGCCGC 
1201   CACCGCGGTG GAGCTTT 

Assembly sequence for sample 77-2-11-A11, Homing endonuclease,from R to F: 
Composition  448  A; 299  C; 337  G; 277  T; 0 OTHER 
Percentage:  33%  A; 22%  C; 25%  G; 20%  T; 0%OTHER 
Molecular Weight (kDa): ssDNA: 421.72    dsDNA: 839.0 
1      TGATGAAGAT ACCCCACCAA ACCCAAAAAA AGAGGGTGGG TCGACTCACA AGTTTGTACA 
61     AAAAAGCGGC TTGTCGACTC GGTGAAATTC TCAGACCAGC GGAAGACGAA CCACTGCGAA 
121    AAGCTTTGCC AGGGATGTTT TCACTGATCA AGAACGAAAG TTAGGGGATC GAAGACGATC 
181    AGATACCGTC GTAGTCTTAA CCATAAACCA TGCCGACTAG GGATTGGAGG ATGTTCCATT 
241    TGTGACTCCT TCAGCACCTT TCGGGAAACT AAAGTCTTTG GGTTCCGGGG GGAGTATGGT 
301    CGCAAGGCTG AAACTTAAAG GAATTGACGG AAGGGCACCA CCAGGAGTGG AGCCTGCGGC 
361    TTAATTTGAC TCAACACGGG GAAACTTACC AGGTCCAGAC ATTGTGAGGA TTGACAGATT 
421    GAGAGCTCTT TCTTGATTCG ATGGGTGGTG GTGCATGGCC GTTCTTAGTT GGTGGAGTGA 
481    TTTGTCTGGT TAATTCCGTT AACGAACGAG ACCGCAGCCT GCTAAATAGC GACGCGAACC 
541    CTCCGTTCGC TGGAGCTTCT TAGAGGGACA ACTTGTCTTC AACAAGTGGA AGTTCGCGGC 
601    AATAACAGGT CTGTGATGCC CTTAGATGTT CTGGGCCGCA CGCGCGCTAC ACTGATGCAC 
661    TCAACGAGTC TATCACCTTG ACCGAGAGGT CCGGGTAATC TTTTGAAATT GCATCGTGAT 
721    GGGGATAGAT TATTGCAACT ATTAATCTTC AACGAGGAAT TCCTAGTAAG CGTGTGTCAT 
781    CAGCGCACGT TGATTACGTC CCTGCCCTTT GTACACACCG CCCGTCGCTC CTACCGATTG 
841    AATGATCCGG TGAGGCCCCC GGACTGCGGC GCCGCAGCTG GTTTCTCCAG CCGCGACGCC 
901    GCGGGAAGCT GTCCGAACCT TATCATTTAG AGGAAGGAGA AGTCGTAACA AGGTTTCCGT 
961    AGTGAACCCT GCGGAAGGAT CATTACCGGT CTTTCCAACC CACACCGATG TTGCACCGTA 
1021   CCCCGTCTAA CCCCGTCGCG GGCTCCCGGT CTCGCGTGGA GCGCGGGCTG TCCGGGGGGG 
1081   CTCTCCTGAG CCGAGAAAAA AAGAAAAATA AAAATAAATA GGAAAAAAAA AAAAAAAAAA 
1141   AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
1201   AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
1261   AAAAAAAAGG GCGGCCGCAC GCGTACCCAG CTTTCTTGTA CAAAGTGGTG ACGTCGAGCT 
1321   CTAAGTAAGT AACGGCCGCC ACCGCGGTGG AGCTTTGGAC T 

    

Assembly sequence for sample 77-2-18-B02, PEPCK, from R to F: 
Composition  287  A; 535  C; 469  G; 236  T; 0 OTHER 
Percentage:  19%  A; 35%  C; 31%  G; 15%  T; 0%OTHER 
Molecular Weight (kDa): ssDNA: 470.49    dsDNA: 941.6 
1      CGATGATGAA GATACCCCAC CAAACCCAAA AAAAGAGGGT GGGTCGACTC ACAAGTTTGT 
61     ACAAAAAAGC AGGCTTGTCG ACTCGCATGA TCAACCGCGA GCGCGCGATC GACTACCTCA 
121    ACACGCGCAA GTCGGTGTAC GTGATCGACG GGTACGCCGG CTGGGATCCC GCCTACCGCG 
181    TGCGCGTGCG CGTCGTCTGC GCCCTCGCCT ACCACGCGCT CTTCATGCGC AACATGCTGG 
241    TGATGCCCGA GGAGCTGCCC GAGGACGAGC GCGAGGAGGG CGACTTCGTG CCCGACTTCA 
301    CGATCCTGAA CGCGGGCGGC TTCCCGGCCA ACCGGTACGT CGAGGGCATG ACCTCCTCCT 
361    GCTCCGTCGA CCTCCACCTC GGCCGCATGG AGATGGTCAT CCTCGGCACG CAGTACGCGG 
421    GCGAGATGAA GAAGGGCATC CTCACGCTCG TCATGTACAC GATGCCGCTC GCCAAGCAGC 
481    TGCCGCTGCA CTCCTCGGCC AACGAGGACG CCGACGGCAA CGTCACCCTC TTCTTCGGCC 
541    TCTCGGGCAC CGGCAAGACC ACCCTCTCCG CCGACCCGCG CCGCAACCTC ATCGGCGACG 
601    ACGAGCACGT CTGGACCGAC CGCGGAGTCT TCAACGTCGA GGGCGGCTGC TACGCCAAGT 
661    GCGTGGGCCT GTCGGCGGAG AAGGAGCCCG AGATCTTCGG CGCGGTCCGG TTCGGCTCCG 
721    TCCTCGAGAA CGTCATCATG GACCTCGACG CGCGCTCCGT CGACTATGAC GACATCTCGG 
781    TGACCGAGAA CACGCGCTGC GCGTACCCGC TCTCGTACAT CCCAAACGCA AAGTCGCCGG 
841    CGGTGGGCGG GCACCCGAAG AACGTGGTGC TGCTCACGTG CGACGGGTAC GGCGTGCTGC 
901    CCCCCATCTC CAAGCTCACC CCCGACCAGG TCATCTACCA CTTCATCGCT GGCTACACCT 
961    CAAAGATGGC CGGCACCGAG GAGGGCATCA CGAAGCCGGT CGCCGCATTC TCCGCGTGCT 
1021   ACGGCGAGCC GTTCCTGGTG TGGCACCCGA TCAAGTACGG CACGATGCTC GCTGAGCGGC 
1081   TGAAGCAGCA CGGCGCCAAC GCCTGGCTCC TCAACACCGG CTGGGTCGGC GGCGCGGCGA 
1141   AGGGGCGGCG CTGCCCGCTG CAGTACACGC GCGCGATCGT CGACGCGATC CACGCTGGCG 
1201   AGCTTGCGAA CGCGCACTGC GACACGGAGC CCTTCTTCGG GCTCAAGTAC CCGACCAAGG 
1261   TGAAGGGCGT GCCCGACTCG CTCCTCAACC CCGCCGCCTC GTGGGAGAGC CAGGCCGACT 
1321   TCGAGGCGAC CTCGAAGGAG CTCGCCGCCC TCTTCAACAA AAACTTTGAG CAGTACTTGG 
1381   GCGACGTGCC CGAGGCGGTG GCGCAGCAGG GCCCCGGGCA GACCAAGATG GCCTACCCCG 
1441   CGGCCGCACG CGTACCCAGC TTTCTTGTAC AAAGTGGTGA CGTCGAGCTC TAAGTAAGTA 
1501   ACGGCCGCCA CCGCGGTGGA GCTTTGG 


    
Assembly sequence for sample 77-2-6-B05, no significant match, from R to F: 
Composition  249  A; 451  C; 495  G; 276  T; 0 OTHER 
Percentage:  17%  A; 31%  C; 34%  G; 19%  T; 0%OTHER 
Molecular Weight (kDa): ssDNA: 455.04    dsDNA: 907.1 
1      GATGATGAAG ATACCCCACC AAACCCAAAA AAAGAGGGTG GGTCGATCAC AAGTTTGTAC  
61     AAAAAAGCAG GCTTGTCGAC TCGCTCGCGC GGCGGCCGAC GAACGTGGAC GAGATGCGCG 
121    CGGTGGCGCT CTCCGCCTTC GTCGCCGCCA TCTTTGAGGA CGCCGCCTGC GCGCGAGGCA 
181    GGTCGCTGCG GCAGTGGCTC GAGGAGCGCT CTCTGCCTCT GCCGGGCGAG TCGAGGGCGG 
241    CTGCTGGAGG CGCGCCGGCC TCGTGGATGG ATTGCGCCTT CCGGACGCCC GCCACGATCG 
301    GGCACGACAG CGGAGAGACG CTGAGGATGC ACATGCAGGA GGGTGCGAGC GGAGAGAGGG 
361    CGAGGGGCGA CCGGGTGCGG CCGGCAGAGC CGCCGCCGCG CGTGCCTCTG CCTTCCCTCC 
421    GCGCGATGGG CTGGGCGCTC CAGCGGGGCG CATCGACGCT GCTCACAACG CCCGTCGGGT 
481    CGATCCTGCT GCCCGCATCG CAGAGCGGCA AGGAGGCGGC GCTCTCGCTC GACCAGTGCT 
541    GCCTCGAGCG ATGGGAGAAT GTGCTGCGAA TCGGGCCGGT GACGGTGCCG ATGTGGTGGC 
601    TGGTGCTCAC GGCGAGCGGC CTGATGTTCG TCGCCTTCTT CCAGTTCTAC CTCTCCTTCG 
661    CCCTCGGACT CGCCTACCGC TCGTGGGAGT ACGTGCTGCA GGCCTCCTGC ATTCTCACGA 
721    TCTTCGCGTC GGCCGGCGCC GTCCTCACCT TCCTCGTACG AGCCTCGAAA GCCGCCGCTC 
781    CGCTCTCCGT TCGGCCTCAC GCCGCATGAC GTCGACATCG CCGGCCGAGT GCAGCCCGAG 
841    GGGTACGGCA TCAAGGGGCT GCTGCTGATG CGGCTGTCGG CACACGCCAT CTACGGGTTC 
901    GTGGTGCTCC TGATGTTCGA ATTCTTCGAC GACGTGCTCA TCGCCGAGGG CCTCGACAGG 
961    GAGGCGGAAT GGGCACCCTT CAAGCAGGCG GGGCTGCTCG GTGGGTACCA TGACCTCCCT 
1021   CCCACCGCCG GTGGAACGTA ATTTCGGTCG TCGTCTTCAT CAAGATGTGC GAGATGCAGT 
1081   CCTCCTTCGC GCCAACCTCG CAGGTCTTCC ATATGTGGCA CCGCGGGCGT GGGCTTCCCG 
1141   CCTTTTGGCC GCGCAGTGAG AAGGACACCT TCATCGAGTG AGGGCGGTAC GTGAGAGGAC 
1201   GGGGCGCGCT CTCTGTGGGA CACACAAACG CAGAGCGCGA TTTTTTTCGT GCCCCCCGGG 
1261   GGGGGGGGGC AGGAGCGTCA ACGTTCAAGT TTCTCGTATC TTGTGCGTGT CTGTGAAATG 
1321   GGTAACGAGT GCTATCAAAT GTAAAAAAAA AAAAAAAAAA AGGGCGGCCG CACGCGTACC 
1381   CAGCTTTCTT GTACAAAGTG GTGACGTCGA GCTCTAAGTA AGTAACGGCC GCCACCGCGG 
1441   TGGAGCTTTG GACGTCTTTC GCCAGAGGTT T 


    


Assembly sequence for sample 77-2-F10, MAPK,from R to F: 
Composition  345  A; 469  C; 410  G; 248  T; 0 OTHER 
Percentage:  23%  A; 32%  C; 28%  G; 17%  T; 0%OTHER 
>Molecular Weight (kDa): ssDNA: 453.81    dsDNA: 907.6 
1      TGAAGATACC CCACCAAACC CAAAAAAAGA GGGTGGGTCG ATCACAAGTT TGTACAAAAA  
61     AGCAGGCTTG TCGACTCGGT CAAGGGCACC ACGTTTGAGG TGGACGTGCG TTACGAGGTG 
121    CGGAAGGGGG TCGGGCAAGG CGCGTACGGA TTGATATGTG CCGCAAAGGA CACAAAGGAC 
181    GGGGACCAGG TCGCGATTAA GAAGATCACA AACGCCTTCG AGGACGCGGT CGACTGCAAG 
241    CGGATGCTGC GGGAGATGCG GCTGCTGCAG CACTTTAAGC ACGAGAACGT TCTCTGCCTC 
301    CGCGACATCA TGAACCCCGA CAAGTCAATC GACGAGTGGA AGGACGTCTA CCTCGTCACC 
361    GAGCTGATGG ACACCGACCT CCATTACATC ATCCACTCGA AGCAAGCGCT CTCGGACGAC 
421    CACATCCAGT ACTTCATGTA CCAGATCCTC CGCGGCCTCA AGGCGGTGCA CGCCGCAAAG 
481    GTGCTCCACC GCGACCTCAA GCCCGGCAAC CTCCTCGTCA ACAAGAACTG CGACCTCAAG 
541    ATTTGCGACT TTGGGCTCGC GCGCGGCGTC GACCCCTCCC AAAAGGGCGT CCAGCAGGCG 
601    GCCCTCACTG AGTACGTGGT GACGCGCTGG TACCGCGCGC CCGAGCTGCT CGTCGAGAAC 
661    GAGACGTACG GCCCGGGCAT CGACATCTGG TCCGTCGGCT GCATCCTCGC CGAGTTCCTG 
721    GGCCGAAAGG CGCTCTTCCC CGGCCGCGAC TATCTGCAGG CACTCCGCCT CATCATCGAG 
781    GCGCTCGGAC CGCCCTCGGA CGAGGACCTC AACACCATCA ACAACCCGCA GGCGGTGGAG 
841    TACATCAAGG CGTTGCCAAA GCGCAAGGCC GTCAACTTTG CGAGCCTCTA CCCGAACGCG 
901    AACAAGGCGG CCATCGACCT TCTGCAGCGG ATGCTGGCGT TCGACGCCCG AAAACGCATC 
961    TCGGCGGCGG AGTCGCTCGA GCACGAGTAC TTGGTGGCGC TGCACAACGT CAACGACGAG 
1021   CCGGACGCGC CCCCGTTCGA CTTTCACTTC GAGAAGGACG ACATCACGGA GCGGCAGCTG 
1081   CGGGGCCTCA TCTGGGACCA GCTGCGCAAC TTCCACCCCG AGGTGCGGAG CGCGGCGGAG 
1141   CTACAATCAG TCTGAAGCGC CCCACAGCCC GCCCCCAGGG CTTGGAGCTT GCGCTCAAGC 
1201   CACCGAGGTG TGACCCGTGT TTTTTTAGTC TCTTCTCTCC CACGTCCCTT CCACCCAGCC 
1261   ACATACACAC ATATTAACCT CAACAGCAAC CCCCTCTCAG TCGTCGGAAC ATAGTGTCTC 
1321   GGAGATCCTG TGCATTGCAT TTGTTTTGTC TAAAAACGCC GAAGAAAAAA AAAAAAAAAA 
1381   AAAAAAAGGG CGGCCGCACG CGTACCCAGC TTTCTTGTAC AAAGTGGTGA CGTCGAGCTC 
1441   TAAGTAAGTA ACGGCCGCCA CCGCGGTGGA GC 


    
GABA Transporter 
Assembly sequence for sample 77-2-19-F11, from R to F: 
Composition  333  A; 524  C; 499  G; 288  T; 0 OTHER 
Percentage:  20%  A; 32%  C; 30%  G; 18%  T; 0%OTHER 
Molecular Weight (kDa): ssDNA: 507.39    dsDNA: 1013.7 
1      ACCGCGGTGG CGGCCGTTAC TTACTTAGAG CTCGACGTCA CCACTTTGTA CAAGAAAGCT  
61     GGGTACGCGT GCGGCCGCCC TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 
121    ATCACATTCA GGCCCATCGA GTATAGACGA AAAATACGAC ACGACACAAC ACGCCGGTAT 
181    TGTCTCCTTG GGGTTGGGCC TTCCCTTGTC TGTTGACAGT TGAGTGTTGT CTCTCTCTCT 
241    GCGTCTCCGA GACGCGAGAG AGAGAGACAG AGAGAGGCGC GGAGGCCAAT TCCCCTAACG 
301    CGTGCGCCAC ACACACCCGG CCACGAGCTA GCCTGTACGG TGCAGCGCCT GCACCCACAT 
361    GCACATGCTC ATGTGGCAGA CTAGCGACCA AGGCCGCGAT TGGAGTTATG TTGGAGTTAT 
421    GTTTGCGCCG CTGCTCGGCT CCCGCGTGGC CGGCAGCTCG GCCGCGCCCC ACAGAGACAC 
481    CGCGCTGCCG CCCTTGCCTT TGCCCTCCTC GACGGCCCCG GCCTCTACCT CACCGGGGCT 
541    GGCGCTCAGG CGGCCCTCGG CACCGAACTC CGCCTCCACG TCGCCAGTTG GCAGCAGCGC 
601    GACGACCAGC GCGGCCAGCA GCCGGAAGCA GGCCGAGGGT CCAGCCGACC CACACGCTCC 
661    AGGCGGGCAG GAAGCCCTTG CCCGCGGGGA AGGGCGTCGA CGACGCCGCG TCCATGATGT 
721    CCCATTTGTT AAGCGACATG GCGACGACGA CGACGAGCAA GGCGGGGCAG ATGAACTTCC 
781    AGCTCACCGT GTGCGTCCAC CCCACCGTCT GGCCCGTCCA CTCCTTCACC CGCCGCCGGA 
841    AGCCGAGCCA GACGGAGCTT CTGAACCAGC ACACGCCGAA GCACTCCATG CCCGTGACGA 
901    AGAACATGGC CACCACGCAG GAGTAGCTGT CCATCAGCTC GAACCAGTAG ATGCCACTCC 
961    GAGTCACAAA GATCAAGCCG AGGAGGTAGG AGAGGACGCA CACCACGGCC GACAGCTTTG 
1021   GCCGCGACAG CCCGCCGCCG ACGCCCGCGT CGCTCAGGAC GGTGACGATG CTCTCGACCA 
1081   TCGCGAACTG CGAGTCGATC CCGAGGCAGA GGAGCATCAC GAAGAAGAGC ATCGCCCAGA 
1141   AGAACCCGCC CGGCATGCTC GCGATGGCGA TGGGGAAGGC GACGAAGGCC AGCCCCGTGC 
1201   CCGACAGACC GACGGACGCC AGCCCGCTCA GGTCATCGCT CCGGATGCAC GGGTTGTACT 
1261   GCGAGAGCTC GTGCGCCAGG TAGCCGAGCA CCGGGAAGAC GACGAAGCCG GCGAAGATGG 
1321   AGGTGCCGCA GTTGATCAAG GACACCTTGG TCGCGTCGCT CAGAAAACGG TTGTGCTTGC 
1381   TCCCGTACGA GGCGAAGGCG ATGAGCGAGC CGAAGCCCAC CCCGAGGGAG TAGAAGATCT 
1441   GCGTCACCGC GCGGACCCAG ACGGTAGCGT CGAGGAGCAC GCTGAAGTCG GGCTTGACGA 
1501   AGAAGGTGAC GCCCGTCGCC GCGTTGGGCA GCGTGATGGC GCGGAAGAAG AAGACGACGA 
1561   GCGAGTCGAC AAGCCTGCTT TTTTGTACAA ACTTGTGATC GACCCACCCT CTTTTTTTGG 
1621   GTTTGGTGGG GTATCTTCAT CATC 

    
Assembly sequence for sample 77-2-12-G01, No Significant match,from R to F: 
Composition  272  A; 318  C; 355  G; 234  T; 0 OTHER 
Percentage:  23%  A; 27%  C; 30%  G; 20%  T; 0%OTHER 
>Molecular Weight (kDa): ssDNA: 364.97    dsDNA: 726.9 
1      CCCACCAAAC CCAAAAAAAG AGGGTGGGTC GATCACAAGT TTGTACAAAA AAGCAGGCTT  
61     GTCGACTCGC TTTGCTCGTC ACATCGCGTT CTCAAGGTGT CCGTCCGTCA ACTCGAGGTG 
121    CAAGATGATG ACAAAGATGA CGCTGCTCAC GCTGATCGTG TGCGCGGGCG CCGTGCCCGT 
181    CGGCCCCAAC TCGGAGGTTG TCCAGAACTC GGAGCTTGCT GGCGACGCGC AGCCCGGTCT 
241    CGCCCTGCAG TTCGGTCCCG GTCCTACTCT GGGCGGCAGT GGCGACTATG ACTGCGGGTG 
301    GAGCTCTGCA GACTTTGGCG AGTATTGCAC CGTGCCGCTT ACCGACGCGC AAGCAAAAGG 
361    CCATCTACAA CCACTACAAA CCACCCCTGG GGGGTGATGA TTCAGTGGAG GGATGGAACA 
421    CGAATTGCAT GAGGTATTGC ACCAAGGACC AACCTTCTAA TTCTAAGTGC GGAAATGGAA 
481    TGCACGGCCA ATGCTACGGG GGAGATGTGT GCGTCAGCCG TCACATCGTC ACAGGTGCCT 
541    GGTACAGCAA GTGCAAATCG ACAGATGGCA TTCCGGAGTT TGATCCACCG ACTCCCCCGT 
601    CTCCGGATGA GTGCATTGCT AACCACTGCA AGGGCGGTTG CCTCCAGCCG GATACGCAGA 
661    ATCCGGGCAA GCAGGTTTGC CACACCGAAG GCCTTGGTCC GGATGCAGAG GATCACTGCA 
721    AACAACATCA CTTTACGTGG TGCCCGGCGG AGTGAAGGCA TCGCCGCATG CAGCATGCAA 
781    CCGCGTGCCA TCGTCTCTAA CGACCCATCG GACCCATCCG ACCAAGATAT GGGGGCGAGA 
841    TGAGTGGGCG CTGAACACTA ACTGTGCGTG AGGGTGGATG GGTACTCGGA ATGAGGAGTG 
901    TCTGATACGT TGTGGCAGTC TAGCGTGCGT GCAGCGGTAG CTCAGAGCCA GCTCTTGTCT 
961    GTCTGGGTTG CAGTATCGGG GCGCGGGATC GGGGTTCGGC GCGGGGTCGT GATGTTCCGG 
1021   GCTCGCTGTC CATTCCCCTC GCCTAACGTG ACGAAATGGG TACTACATGT TTATATACAA 
1081   AAAAAAAAAA AAAAAAAAAA AGGGCGGCCG CACGCGTACC CAGCTTTCTT GTACAAAGTG 
1141   GTGACGTCGA GCTCTAAGTA AGTAACGGCC GCACCGCGG 

    
MYB 
Assembly sequence for sample 77-2-35-H10, cmyb from R to F: 
Composition  227  A; 429  C; 398  G; 176  T; 0 OTHER 
Percentage:  18%  A; 35%  C; 32%  G; 14%  T; 0%OTHER 
>Molecular Weight (kDa): ssDNA: 379.48    dsDNA: 758.5 
1      TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGG GTGGGTCGAT CACAAGTTTG  
61     TACAAAAAAG CAGGCTTGTC GACTCGCATG CACATCCCTC CCGCGGTGCC GCAGCGGTCC 
121    CTCGCGGCGG CGGTCACCGT GCCGAATCTG CTCCCTCCCG CCCGCTCGCC ACACGGCCGG 
181    CCGAGCGCCA CGGCGGTCGA GATGGAGCTC GGCGGCCGGC GGGGCAAGGA GGTGGAGCGG 
241    AAGGAGTGGC TGGCTTCCGA GGATCAGATT ATCCGCGACG GAGTGCAGCG CCTCGGCTGC 
301    CGCTGGCGGC GCATAGCGGA GCGGCTTCCG GGCCGGTCGG ACGACGCGGT GCGAACCGGT 
361    GGAACCGCTG AAGGAGATGG AGCGCAAGGG CCCCGACGCG TGCGACGCGG CGCCCGACGA 
421    GCTCAGCGAG TCGAGCGGCG GCGCTGCGCG GCCGGCCGCC GCCTCGACCT CCTGCGACGG 
481    GCAGCGCGAG GAGGAGGGGG CGGGCAAGAC CGAGCGTGTG AGCTGGACGA CGGAGGAGGA 
541    CGCGATCGTC GTCAACTCGG TCAAGGCGCA CGGGCACAAG TGGAGCCAGA TCTCCCAGTC 
601    GTTGCCGGGC CGGACTGACC ACGCCATCCG CAACCGGTGG CAGCGGCTAC TCAAACTCGA 
661    GAACCAGGCG CGAAATGGCC ACGCCGCAAA GACGAAGCGC TTCCCCTCCG CCGACCACCC 
721    GGTGCAGAGG GGCCACTTCC ACCCGCGCTT CCCGCAGCTG CCGTCGCAGC TACCGCCGCA 
781    GCTGCCGCCC CCGCAGCTCC AGCTGCCCAC GTCGGGAAGG CGTTGCCGGG CCCGCTCTTC 
841    ACGGGCGCCG AGGATGCACG CGACGGCAGC TACGAGCTGC CTTGAGCCCG CGACTCGTGG 
901    TGTTCACGCG TCCGACGCCT CGCTCCTCCG CCTCCCCGCT CTCGCTCCTC GCGCGCGCTC 
961    TCTCTCCACG GACCAGTGCC AGTGGTCCGT GCGCACTCTG AAGCCCTCCC AGTCGTCCTC 
1021   CCCCCGTCTC TCTCGTCTGT TTGGCGTGTC TATTGCCCCT CTCTCCGCGT CTGCGGGCCG 
1081   CGCGTCCGCG CGGTCCACGT TCTCAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
1141   GGGCGGCCGC ACGCGTACCC AGCTTTCTTG TACAAAGTGG TGACGTCGAG CTCTAAGTAA 
1201   GTAACGGCCG CCACCGCGGT GGAGCTTTGG 


    
Assembly sequence for sample 77-2-28-E06, metalloproteinase: 
Composition  285  A; 342  C; 344  G; 163  T; 10 OTHER 
Percentage:  25%  A; 30%  C; 30%  G; 14%  T; 1%OTHER 
>Molecular Weight (kDa): ssDNA: 353.86    dsDNA: 705.4     
1      TCGATGATGA AGATACCCCA CCAAACCACA AAAAAGAGGG GTGGCTGATC ACAAGTTTGT  
61     ACAAAAAAGC AGGCTTGTCG ACTCGCCTCC TGCAGCTCAA ACAATGCTCG CCACCTCGAT 
121    GCTCTCGCTC TCGGCCAACC GCGCGGAGGT CTCGACCGGC AACCTCCGTG GCGACGCGGG 
181    GAAGTTCGAT AACCTCAAGG CCTCGTGGGA GAAGGGGCTG GAGATCGGCG ACTTCAAGAC 
241    CAAGGTGGCG GCCAAGTACG ACTACAACTC GAACAAGGAC TTCCTCAAGG AGGTCTCCTT 
301    CTCGGGCGAC CTGGTCGAGG GCGACTCCGC GGACGACGTC TCGGTCTCGT ACGAGCTGCG 
361    GCGCGACTTT GCGAGCAAGC GGTCCGACGT GCGGCTGACG GCCTCGTCGC AGGGCACGAC 
421    CCTGTCGGCC GAGTACGACA CGGAGGACCA GCTGACGGAG GTTGCGGTGC AGCGCGAGGT 
481    CGACCTCGGC GACCAGAAGG TGGACGTGCA GCCGAGCTGG ATGGTCAAGG CCAAGGCTGC 
541    GCGCGTCAAG CTCATGTCCG GCCTCAACAA CGGCAAGGAC AAGGTCTCGG CGCAGTTCGA 
601    CTACGACGTC GACGGCAAGG AGGTCGGCGC GCTCGAGGTC GGCTTCACCC GCCAGCTCAA 
661    GGCGGGGCAG ACGCTCGCCG CCTCGTACAA GCCCGACAAG TCGGACCTCG AGGTGTCGCT 
721    GCAGGACGAC AACTTTGAGG ACGGCGCGAA CCTGGACCGC GACCGCCAAC GTGCCGCTCG 
781    AGTCGGCCGA CGCGAACCTC CTCGACGCGG CGCGCGTCAC CCTCAAGCGC TCCTGGGGCT 
841    GGTAGACCCG CTCGCCCTCC GGCCCTCCCC TGCCGCAGTA GAGGGGCGAC GGCACAGCCG 
901    CGAGAGCCGC CCATCTGTTG ACCCCTCAAC AGTGACGACT CAGATGTGTG CGTGTGTATG 
961    TGCATGGATK RAAAGCAKGA WWCMCCKKTY SCAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
1021   AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAGG GCGGCCGCAC GCGTACCCAG 
1081   CTTTCTTGTA CAAAGTGGTG ACGTCGAGCT CTAAGTAAGT AACGGCCGCC ACCGCGGTGG 
1141   AGCT 

    
Assembly sequence for sample 77-2-1-H3, Pumilio: 
Composition  307  A; 516  C; 493  G; 263  T; 9 OTHER 
Percentage:  19%  A; 32%  C; 31%  G; 17%  T; 1%OTHER 
>Molecular Weight (kDa): ssDNA: 490.14    dsDNA: 979.2     
1      TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGG GTGGGTCGAT CACAAGTTTG  
61     TACAAAAAAG CAGGCTTGTC GACTCGCGTT AATGAGTGAA CGGCCAGTAC TTTACTAGGC 
121    GACCAAGGAG GACGTCGTCT CCCTCGGCAT GGACCCGTTC GGCAACTACC TCGTGCAGAA 
181    GCTAGTGCAG TACGGCACCG GCGAGCAGCG CGCCGCACTC ATCGACGGCA CGTCGAGCGG 
241    ATGGTCGAGG TCGCGCTCAA CGTGCACGGG ACGCGCGTGG TGCAAAAGAT GGTGGAGTGC 
301    GCCGACAGTG CCGCCCAGCA GTCGGCCCTG TGCGCCGCGC TCTTGCCCTC GATCATGCAG 
361    CTCATCCGCG ACATGAACGC GAACCACGTG GTGCAGCGCT GCCTCGCCGC GCTGCCCGCC 
421    GAGCTGGCCG CGGGCATCTA TGACCGCGCC GTCTCGGATT GCCTCGCCAT CGCGACGCAC 
481    CGGCACGCTG CTGCGTGCTG CAGCGCTGCC TGGACCACGC CCCGGCGCCG CACCGCGAGG 
541    AGATCATCGG CGAGGTCGTG CGGCTCGCGC CCAAGCTCGT CGTCGACCCG TTTGGCAATT 
601    ACGTCGTGCA GTACATCCTC GACTTGCGCC AGCGCCGCTC ACGCACGCGA TCGTGCAGGG 
661    CGCTGCGCGC CGGCCTTCGC GGAGCTCTCC CTCCAAAAGT TCTCCTCCAA CGTGATCGAA 
721    AAGTGCCTCA CGTCGGGCGA CCCATCTCTC CCTCCTCTCT CCCCCATCTC TCCCCATCTC 
781    TCCCCATAGG TGCCTCAAGT CGGGCGACCC GACCGCAGTC GCGGTGGTCG CCAACGAGCT 
841    GACCGCGTCC GAGGCGTTCG CCACGCTGCT GCACGACCCG TTCGCAAACT ATGTCATCCA 
901    GACCCTCCTC ACCGTCGGCA AGGACGACGT GGTCGCCCTG CTCGTCGCAC AGATCACGCC 
961    GCACCTGCCC ACCCTCCGCT CGACCCTGTA CGGAAAGCGC ATCCAGGCCA AGCTCCTCAA 
1021   GCGCTGCCCC SSCMMMAARG CSGCGCTGCC CCAGCGCTGA CGGCGCGGAC ACGGCGCGGA 
1081   CACGGCGGAT GGTGCGGTGC GGAGACGGCG CGGATGGCGC GGAAGCTGCG CTGACGGCGC 
1141   GSYTGCGCGG CGGGGGAGTG ACACATTAGG AGAGAGGCGG TGCTTGCGGG GGCGGGGCGG 
1201   GGTCGAGGAG TTTTGGGGTG CACATTTGCT GCGGGCGCGG TGCCTTCGCC CCTTTGCGCA 
1261   TGCCGTGTGC CGCTTGGGGC GCACCACGAG AACAGCATGG CGGGCCGCGG AATTGTGCGC 
1321   ATGAGTACTA TGTCGCGGGG AAATCTCGCA AGCGCCTCTC GAGTCCGGTG CGTGGGCGCG 
1381   GCCCCGCGCT GCGGCCGAGT GGGTCTTGCT TGATGTGTGG CTTTCTCGCG GGTTTTTAAA 
1441   CGCAGAACAA TCGAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 
1501   GGGCGGCCGC ACGCGTACCC AGCTTTCTTG TACAAAGTGG TGACGTCGAG CTCTAAGTAA 
1561   GTAACGGCCG CCACCGCGGT GGAGCTTT 

Ambiguity coding: 

A Adenine
C Cytosine
G Guanine
T Thymine (Uracil) 

a	Most likely A 
c 	Most likely C 
g 	Most likely G 
t 	Most likely T 

R	A or G
S	C or G 
W 	A or T 
Y 	C or T 
K 	G or T 
M 	C or A 

B 	C or G or T
D 	A or G or T 
H 	A or C or T 
V	A or C or G 
N 	Either A, C, G, or T

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